{"text_format":"1","workbook":"xlsx","n_sheets":1,"sheets":["Sheet 1"],"not_rendered":{"charts":0,"images":0,"macros":1,"external_links":0,"defined_names":2,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"Sheet 1","index":0,"n_rows":86,"n_cols":49,"rows_kept":86,"cut":false,"hidden":false,"merged":[[50,3,50,7],[12,15,12,16],[13,15,13,17]]}
["Directions: From left to right, enter information for each sample and follow the recommended pooling guidelines. ",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"PN40676RevL"]
["Do not enter anything in cells that are red. Blue columns indicate pipetting steps.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"2024-03-25"]
["[v2 Chemistry] Recommended values for expected # cells: 5,000 for whole cells; 3,000 for nuclei; 1,000 for low (20,000 cells) input."]
["[v3 Chemistry] Recommended values for expected # cells: 11,000 for whole cells; 8,000 for nuclei; 3,000 for low (30,000 cells) input."]
["Click here for more information on sequencing coverage requirements; contact illumina support for secific sequencer, capacity, and loading questions."]
[]
[]
[]
[]
["Sequencing Instrument -->"]
["Max read pairs ",{"$f":"IFERROR(VLOOKUP($B$10,$W$20:$Z$37,3,0), \"\")","v":""}]
["% PhiX",{"$f":"IFERROR(VLOOKUP($B$10,$W$20:$Z$37,2,0)-1, \"\")","v":""}]
["Patterned",{"$f":"IFERROR(VLOOKUP($B$10,$W$20:$Z$37,4,0),\"\")","v":""},null,null,null,null,null,null,null,null,null,null,null,null,null,"                        Target Molarity -->",null,null,5,"nM"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"                              Target Volume -->",null,null,30,"uL "]
[]
[null,"Sample Name","Library Type","Index","Additional Indexes i7","Additional Indexes i5","Panel Type","Panel Size ","Custom Info","Expected # cells","Reads Required","Qubit (ng/uL)","Avg. Size (bp)","Molarity (nM)","Volume of Sample (uL) ","Volume of Water (uL)","Final Molarity (nM)","Volume to Pool","Volume to Pool (uL)"]
["Sample 1",null,null,null,null,null,null,{"$f":"IFERROR(VLOOKUP(G17,$AE$21:$AF$31,2,0),\"\")","v":""},null,null,{"$f":"IF(ISBLANK(J17),\"\",IF(AND(C17=$AB$21,ISNUMBER(H17)),H17*J17*$AI$20,IF(G17=$AB$27, J17*I17*$AI$20,IF(AND(C17=$AC$21, ISNUMBER(H17)),J17*H17*$AI$21,IF(G17=$AC$24,J17*I17*$AI$21,(J17* (I17+$AF$26)*$AI$21))))))","v":""},null,{"$f":"IFERROR(IF(L17=\"\",\"\", IF(C17=$AB$21,450,IF(C17=$AC$21, 260,\"\"))), \"\")","v":""},{"$f":"IFERROR(L17/($AL$20*M17)*10^6,\"\")","v":""},2,{"$f":"IFERROR(((N17*O17)/$S$13)-O17,\"\")","v":""},{"$f":"IFERROR(N17*O17/(P17+O17),\"\")","v":""},{"$f":"IFERROR(IF(L17=\"\", \"\", IF(C17=$AB$21, $S$14*(K17/SUM($K$17:$K$32)),IF($B$13=$Z$24,$S$14*$AL$21*(K17/SUM($K$17:$K$32)),$S$14*(K17/SUM($K$17:$K$32))))),\"\")","v":""},{"$f":"IFERROR(IF(L17=\"\", \"\",( R17/SUM($R$17:$R$32))*$S$14),\"\")","v":""},{"$f":"IFERROR(IF(N17<$S$13,\"Warning: Molarity too low; lower the target molarity (above).\",IF(S17>(P17+O17), \"Warning: Not enough diluted sample; increase the volume of sample (column O) or lower the target volume (above).\", \"\")),\"\")","v":""},null,null,"Sequencer Info",null,null,null,null,"Panel Options",null,null,"Panel Sizes",null,null,"Coverage",null,null,"Other constants",null,null,"Index sequences"]
["Sample 2",null,null,null,null,null,null,{"$f":"IFERROR(VLOOKUP(G18,$AE$21:$AF$31,2,0),\"\")","v":""},null,null,"",null,"","",2,{"$f":"IFERROR(((N18*O18)/$S$13)-O18,\"\")","v":""},{"$f":"IFERROR(N18*O18/(P18+O18),\"\")","v":""},"",{"$f":"IFERROR(IF(L18=\"\", \"\",( R18/SUM($R$17:$R$32))*$S$14),\"\")","v":""},{"$f":"IFERROR(IF(N18<$S$13,\"Warning: Molarity too low; lower the target molarity (above).\",IF(S18>(P18+O18), \"Warning: Not enough diluted sample; increase the volume of sample (column O) or lower the target volume (above).\", \"\")),\"\")","v":""}]
["Sample 3",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"Sequencer","PhiX","Max Reads","Patterned?"]
["Sample 4",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"MiSeq | V3",1.05,25000000,"No",null,null,null,null,null,null,null,"DNA",80,"per amp, per cell","Molecular weight per bp",660,null,"DNA","Protein",null,"DNA Additional Indexes","Protein Additional Indexes",null,null,"i7","i5","i5 Reverse Complement"]
["Sample 5",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"HiSeq 2500 | Rapid Run Single",1.1000000000000001,300000000,"No",null,"DNA","Protein",null,"AML",127,null,"per AOC",750,"per AOC, per cell","Adjustment factor for protein libs",0.75,null,"DNA Index 1","Protein Index 1",null,"DNA Unique 9","Protein Unique 9",null,"DNA Index 1","CTGATCGT ","ATATGCGC ","GCGCATAT"]
["Sample 6",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"HiSeq 2500 | Rapid Run Dual",1.1000000000000001,600000000,"No",null,"AML","Heme Oncology 45-plex",null,"MYE",312,null,null,null,null,null,null,null,"DNA Index 2","Protein Index 2",null,"DNA Unique 10","Protein Unique 10",null,"DNA Index 2","ACTCTCGA ","TGGTACAG","CTGTACCA"]
["Sample 7",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NextSeq 1000/2000 | P1",1.07,100000000,"Yes",null,"MYE","Heme Oncology 45-plex + spike-in",null,"AML MRD",321,null,null,null,null,null,null,null,"DNA Index 3","Protein Index 3",null,"DNA Unique 11","Protein Unique 11",null,"DNA Index 3","TGAGCTAG","AACCGTTC ","GAACGGTT"]
["Sample 8",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NextSeq 1000/2000 | P2",1.07,400000000,"Yes",null,"AML MRD","Custom AOC pool",null,"scMRD AML 17-plex",17,null,null,null,null,null,null,null,"DNA Index 4","Protein Index 4",null,"DNA Unique 12","Protein Unique 12",null,"DNA Index 4","GAGACGAT ","TAACCGGT ","ACCGGTTA"]
["Sample 9",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NextSeq 2000 | P3",1.07,1200000000,"Yes",null,"gwCNV","scMRD AML 17-plex",null,"Custom","Enter amplicon # -->",null,null,null,null,null,null,null,"DNA Index 5","Protein Index 5",null,"DNA Unique 13","Protein Unique 13",null,"DNA Index 5","CTTGTCGA ","GAACATCG ","CGATGTTC"]
["Sample 10",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NextSeq 2000 | P4",1.07,1800000000,"Yes",null,"MM","MM 19-plex",null,"Heme Oncology 45-plex",45,null,null,null,null,null,null,null,"DNA Index 6","Protein Index 6",null,"DNA Unique 14","Protein Unique 14",null,"DNA Index 6","TTCCAAGG ","CCTTGTAG ","CTACAAGG"]
["Sample 11",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NextSeq 500/550 | Mid-Output",1.2,130000000,"No",null,"Custom",null,null,"Heme Oncology 45-plex + spike-in","Enter # of AOC spike-ins -->",null,null,null,null,null,null,null,"DNA Index 7","Protein Index 7",null,"DNA Unique 15","Protein Unique 15",null,"DNA Index 7","CGCATGAT","TCAGGCTT ","AAGCCTGA"]
["Sample 12",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NextSeq 500/550 | High-Output",1.2,400000000,"No",null,null,null,null,"Custom AOC pool","Enter AOC # -->",null,null,null,null,null,null,null,"DNA Index 8","Protein Index 8",null,"DNA Unique 16","Protein Unique 16",null,"DNA Index 8","ACGGAACA ","GTTCTCGT","ACGAGAAC"]
["Sample 13",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"HiSeq 3000",1.2,2500000000,"Yes",null,null,null,null,"gwCNV",500,null,null,null,null,null,null,null,"DNA Additional Indexes","Protein Additional Indexes",null,null,null,null,"Protein Index 1","TAAGGCGA ","ATATGCGC ","GCGCATAT"]
["Sample 14",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"HiSeq 4000",1.2,5000000000,"Yes",null,null,null,null,"MM",345,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Index 2","CGTACTAG","TGGTACAG","CTGTACCA"]
["Sample 15",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NovaSeq 6000 | SP",1.1499999999999999,800000000,"Yes",null,null,null,null,"MM 19-plex",19,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Index 3","AGGCAGAA ","AACCGTTC ","GAACGGTT"]
["Sample 16",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NovaSeq 6000 | S1",1.1499999999999999,1600000000,"Yes",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Index 4","TCCTGAGC ","TAACCGGT ","ACCGGTTA"]
["Sample 17",null,null,null,null,null,null,"",null,null,"",null,{"$f":"IFERROR(IF(L33=\"\",\"\", IF(C33=$AB$21,450,IF(C33=$AC$21, 260,\"\"))), \"\")","v":""},{"$f":"IFERROR(L33/($AL$20*M33)*10^6,\"\")","v":""},2,{"$f":"IFERROR(((N33*O33)/$S$13)-O33,\"\")","v":""},{"$f":"IFERROR(N33*O33/(P33+O33),\"\")","v":""},"",{"$f":"IFERROR(IF(L33=\"\", \"\",( R33/SUM($R$17:$R$32))*$S$14),\"\")","v":""},"",null,null,"NovaSeq 6000 | S2",1.1499999999999999,4100000000,"Yes",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Index 5","GGACTCCT ","GAACATCG ","CGATGTTC"]
["Sample 18",null,null,null,null,null,null,"",null,null,"",null,"","",2,{"$f":"IFERROR(((N34*O34)/$S$13)-O34,\"\")","v":""},{"$f":"IFERROR(N34*O34/(P34+O34),\"\")","v":""},"",{"$f":"IFERROR(IF(L34=\"\", \"\",( R34/SUM($R$17:$R$32))*$S$14),\"\")","v":""},"",null,null,"NovaSeq 6000 | S4",1.1499999999999999,10000000000,"Yes",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Index 6","TAGGCATG ","CCTTGTAG ","CTACAAGG"]
["Sample 19",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NovaSeq X | 1.5B",1.1499999999999999,1600000000,"Yes",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Index 7","CTCTCTAC ","TCAGGCTT ","AAGCCTGA"]
["Sample 20",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NovaSeq X | 10B",1.1499999999999999,10000000000,"Yes",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Index 8","CAGAGAGG ","GTTCTCGT","ACGAGAAC"]
["Sample 21",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,"NovaSeq X | 25B",1.1499999999999999,26000000000,"Yes",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"DNA Unique 9","CGGCTAAT","AGAACGAG","CTCGTTCT"]
["Sample 22",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"DNA Unique 10","ATCGATCG","TGCTTCCA","TGGAAGCA"]
["Sample 23",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"DNA Unique 11","GCAAGATC","CTTCGACT","AGTCGAAG"]
["Sample 24",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"DNA Unique 12","GCTATCCT","CACCTGTT","AACAGGTG"]
["Sample 25",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"DNA Unique 13","TACGCTAC","ATCACACG","CGTGTGAT"]
["Sample 26",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"DNA Unique 14","TGGACTCT","CCGTAAGA","TCTTACGG"]
["Sample 27",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"DNA Unique 15","AGAGTAGC","TACGCCTT","AAGGCGTA"]
["Sample 28",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"DNA Unique 16","ATCCAGAG","CGACGTTA","TAACGTCG"]
["Sample 29",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Unique 9","AGCGTAGC","AGAACGAG","CTCGTTCT"]
["Sample 30",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Unique 10","CAGCCTCG","TGCTTCCA","TGGAAGCA"]
["Sample 31",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Unique 11","TGCCTCTT","CTTCGACT","AGTCGAAG"]
["Sample 32",null,null,null,null,null,null,"",null,null,"",null,"","",2,"","","","","",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Unique 12","TCCTCTAC","CACCTGTT","AACAGGTG"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Unique 13","TCATGAGC","ATCACACG","CGTGTGAT"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Unique 14","CCTGAGAT","CCGTAAGA","TCTTACGG"]
["Total read pairs (including PhiX)",{"$f":"IFERROR(IF(SUM(K17:K32)*(1+B12)=0, \"\",SUM(K17:K32)*(1+B12) ),\"\")","v":""},"(2 x 150bp)",{"$f":"IF(B51=\"\",\"\",IF($B$51>$B$11,\"Warning: number of required reads exceeds limit for this sequencing instrument.\",\"\"))","v":""},null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Unique 15","TAGCGAGT","TACGCCTT","AAGGCGTA"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Protein Unique 16","GTAGCTCC","CGACGTTA","TAACGTCG"]
["Index Primer Information:"]
[null,"Sample Name","Index i7","Index i5","Index i5 Reverse Complement"]
["Sample 1",{"$f":"IF(B17=\"\", \"\", B17)","v":""},{"$f":"IFERROR(IF(E17=\"\",VLOOKUP(D17, $AT$20:$AW$52,2,0),VLOOKUP(E17, $AT$20:$AW$52,2,0)), \"\")","v":""},{"$f":"IFERROR(IF(F17=\"\",VLOOKUP(D17, $AT$20:$AW$52,3,0),VLOOKUP(F17, $AT$20:$AW$52,3,0)), \"\")","v":""},{"$f":"IFERROR(IF(F17=\"\",VLOOKUP(D17, $AT$20:$AW$52,4,0),VLOOKUP(F17, $AT$20:$AW$52,4,0)), \"\")","v":""}]
["Sample 2",{"$f":"IF(B18=\"\", \"\", B18)","v":""},{"$f":"IFERROR(IF(E18=\"\",VLOOKUP(D18, $AT$20:$AW$52,2,0),VLOOKUP(E18, $AT$20:$AW$52,2,0)), \"\")","v":""},{"$f":"IFERROR(IF(F18=\"\",VLOOKUP(D18, $AT$20:$AW$52,3,0),VLOOKUP(F18, $AT$20:$AW$52,3,0)), \"\")","v":""},{"$f":"IFERROR(IF(F18=\"\",VLOOKUP(D18, $AT$20:$AW$52,4,0),VLOOKUP(F18, $AT$20:$AW$52,4,0)), \"\")","v":""}]
["Sample 3",{"$f":"IF(B19=\"\", \"\", B19)","v":""},"","",""]
["Sample 4","","","",""]
["Sample 5","","","",""]
["Sample 6","","","",""]
["Sample 7","","","",""]
["Sample 8","","","",""]
["Sample 9","","","",""]
["Sample 10","","","",""]
["Sample 11","","","",""]
["Sample 12","","","",""]
["Sample 13","","","",""]
["Sample 14","","","",""]
["Sample 15","","","",""]
["Sample 16","","","",""]
["Sample 17",{"$f":"IF(B33=\"\", \"\", B33)","v":""},"","",""]
["Sample 18","","","",""]
["Sample 19",{"$f":"IF(B35=\"\", \"\", B35)","v":""},"","",""]
["Sample 20","","","",""]
["Sample 21","","","",""]
["Sample 22","","","",""]
["Sample 23","","","",""]
["Sample 24","","","",""]
["Sample 25","","","",""]
["Sample 26","","","",""]
["Sample 27","","","",""]
["Sample 28","","","",""]
["Sample 29","","","",""]
["Sample 30","","","",""]
["Sample 31","","","",""]
["Sample 32","","","",""]