Step 6: Variant filtering

  • Updated

The following variant filters are applied by the genome editing pipeline:

  • Total read depth (DP) >= 10
  • Alternate allele frequency (AF) >= 10%
  • Genotyping quality (GQ) >= 30

Any variants that fail these filters are converted to no-calls and as a result the cells containing such variants are excluded from further downstream analysis for that particular target.

Share this article:

Was this article helpful?

0 out of 0 found this helpful

Have more questions? Submit a request