Alignment to VCF

  • Updated

This part of the DNA Pipeline processes each tube individually and generates a VCF file as the final output.

The steps involved are:

  • Adapter trimming,
  • Barcode detection,
  • Reference genome alignment,
  • Barcode correction,
  • Fixing mate read information,
  • Cell detection, and
  • Variant calling.

At the end of this step, a VCF file is generated for each tube. This file is then passed to the next step for the .loom file generation.

Share this article:

Was this article helpful?

0 out of 1 found this helpful

Have more questions? Submit a request