What do I do if my sequencing run is overclustered?

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Overclustering on the sequencer is likely due to loading a sequencing library that is too concentrated. 

We recommend re-quantifying the individual tube libraries using Qubit (or a Bioanalyzer for dilute ~ 5nM samples (100 – 700 bp range)), re-normalizing, and re-pooling the library. Before sequencing, the pooled library needs to be re-quantified using either Qubit or qPCR (KAPA KK4873).

For additional information and guidance, refer to the User Guide. 

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