Overclustering on the sequencer is likely due to loading a sequencing library that is too concentrated.
We recommend re-quantifying the individual tube libraries using Bioanalyzer (100 – 700 bp range), re-normalizing, and re-pooling the library. Before sequencing, the pooled library needs to be re-quantified using either Qubit or qPCR (KAPA KK4873).
For additional information and guidance, refer to Chapter 9 – Sequence Library in the Tapestri Single-Cell DNA Sequencing V2 User Guide.