What’s the optimal cell number and concentration?

  • Updated

V2 Chemistry

For DNA-only and DNA + Protein applications, the total number of cells required for cartridge loading is 140,000 in 35 µL.

Note: If fewer than 140,000 cells in 35 µL are provided, the quality of the data may be impacted, such as cell throughput. For DNA-only runs, cell input numbers as low as 30,000 in 35 µL have been validated to have the same capture rate as standard 140,000 cell runs.

A minimum of 35 µL of cell suspension is required for encapsulating cells.

V3 Chemistry

For DNA-only and DNA + Protein applications, the total number of cells required for cartridge loading is 30,000 - 100,000 in 35 µL.

A minimum of 35 µL of cell suspension is required for encapsulating cells.

Cell preparation

To account for sample loss and cell quantification, we recommend starting with a minimum number of cells:

  • 0.5 million – DNA-only applications
  • 2 million – DNA/protein applications

The DNA/protein workflow includes a staining step that requires multiple washing steps prior to loading the cells onto the cartridge for encapsulation. Therefore, we recommend starting with a 4x higher cell number as compared to DNA-only applications.

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