Can I reduce the DNA library concentration to < 5 nM?

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We recommend that the pooled Tapestri library be concentrated at 5 nM before the NaOH treatment (denaturation), following Illumina’s recommendation.

For equimolar pooling, if one of the sample libraries you intend to pool is concentrated below 5 nM (~1.1 ng/µL), it is okay to lower the total concentration of the final library pool to enable equimolar pooling of all sample libraries.

It is critical to follow the final library loading concentration guidelines in Table 7 in the Tapestri Single-Cell DNA Sequencing V2 User Guide, which varies from 2 – 400 pM depending on the sequencer.

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