PhiX recommendations are based on Illumina’s recommendations:
| MiSeq | HiSeq 2500 | HiSeq 3000 | HiSeq 4000 | NextSeq 500/550 | Novaseq 6000 |
|---|---|---|---|---|---|
| 5 % | 10 % | 15 % | 15 % | 20 % |
15 % |
Note: Protein libraries have lower diversity than DNA libraries. It is generally recommended to sequence DNA and Protein libraries together to increase diversity. Increased Phi-X is recommended if sequencing Protein libraries alone.