After Encapsulation is finished, the collection tube is filled with 50 – 70 µL of emulsions and 80 – 120 µL of oil.
Some reasons for low volume include:
- The reagents pipetted onto the wrong DNA cartridge reservoir.
- Insufficient volume of reagents pipetted onto the DNA cartridge.
- Reagents were not loaded onto the DNA cartridge.
- Cartridge clogging occurred due to debris in one of the reagents.
- Bubbles present in one of the reagents after cartridge loading.