What causes low emulsion volume in the collection tube?

  • Updated

After Encapsulation is finished, the collection tube is filled with 50 – 70 µL of emulsions and 80 – 120 µL of oil.

Some reasons for low volume include:

  • The reagents pipetted onto the wrong DNA cartridge reservoir.
  • Insufficient volume of reagents pipetted onto the DNA cartridge.
  • Reagents were not loaded onto the DNA cartridge.
  • Cartridge clogging occurred due to debris in one of the reagents.
  • Bubbles present in one of the reagents after cartridge loading.
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