What causes low emulsion quality, how do I identify it?

  • Updated

The two main sources impacting emulsions quality/stability are:

  • Thermal cycling
  • Emulsion handling

Thermal cycling

  • Incorrect PCR tubes used, other than the 0.2 mL Axygen MAXYmum Recovery PCR Tubes and PCR-02-L-C
  • PCR tubes are not fully closed
  • No skirt or supporting ‘corner’ PCR tubes used for even heat transfer

 A layer of fluid above the emulsion layer after the targeted PCR (1st PCR) is indicative of a failure mode.


If you notice an aqueous layer on top of the emulsion layers after thermal cycling, contact support@missionbio.com

 Emulsion handling

  • Static electricity on tubes, gloves, or devices
  • Mechanical stress on tubes

Emulsion tubes are very sensitive to uneven heating, physical stress, or harsh handling. Please refer to the Tapestri Single-Cell DNA Sequencing V2 User Guide for instructions on the safe-handling of emulsions.

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