If there is excess volume left in the Barcoding Bead reservoir of the cartridge, please contact support@missionbio.com.
- Measure the leftover volume of the other reagents.
- Carefully inspect the emulsions of the 8 sample tubes.
- Move forward with the workflow, and record 1st PCR DNA concentrations (Qubit).
Potential reasons for excess leftover volume of more than 120 μL after the Barcoding program has completed:
| Reason | Suggested Resolution |
| Insufficient vortexing of Barcoding Beads | Vortex Barcoding Beads tube for 1 minute immediately prior to reagent loading. |
| Debris inside the cartridge | Protect the cartridge from its environment at all times when not in use. |
| Instrument-related pressure issue | Export the log files from the Instrument and send them to Mission Bio Support for review. |
| Cartridge-related gasket issue | Verify that the gasket is positioned correctly on top of the cartridge. |
| Cartridge-related issue (e.g., microfluidics channel compromised) | Record the cartridge serial number, keep the cartridge, and contact Mission Bio Support. |