If there is excess volume left in the Barcoding Bead reservoir of the cartridge, please contact firstname.lastname@example.org.
- Measure the leftover volume of the other reagents.
- Carefully inspect the emulsions of the 8 sample tubes.
- Move forward with the workflow, and record 1st PCR DNA concentrations (Qubit).
Potential reasons for excess leftover volume of more than 120 μL after the Barcoding program has completed:
|Insufficient vortexing of Barcoding Beads
|Vortex Barcoding Beads tube for 1 minute immediately prior to reagent loading.
|Debris inside the cartridge
|Protect the cartridge from its environment at all times when not in use.
|Instrument-related pressure issue
|Export the log files from the Instrument and send them to Mission Bio Support for review.
|Cartridge-related gasket issue
|Verify that the gasket is positioned correctly on top of the cartridge.
|Cartridge-related issue (e.g., microfluidics channel compromised)
|Record the cartridge serial number, keep the cartridge, and contact Mission Bio Support.