If there is excess volume left in the Barcoding Bead reservoir of the cartridge, please contact firstname.lastname@example.org.
- Measure the leftover volume of the other reagents.
- Carefully inspect the emulsions of the 8 sample tubes.
- Move forward with the workflow, and record 1st PCR DNA concentrations (Qubit).
Potential reasons for excess leftover volume of more than 120 μL after the Barcoding program has completed:
|Insufficient vortexing of Barcoding Beads||Vortex Barcoding Beads tube for 1 minute immediately prior to reagent loading.|
|Debris inside the cartridge||Protect the cartridge from its environment at all times when not in use.|
|Instrument-related pressure issue||Export the log files from the Instrument and send them to Mission Bio Support for review.|
|Cartridge-related gasket issue||Verify that the gasket is positioned correctly on top of the cartridge.|
|Cartridge-related issue (e.g., microfluidics channel compromised)||Record the cartridge serial number, keep the cartridge, and contact Mission Bio Support.|