Do I need to clean DNA libraries with off-target products?

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DNA-only protocol

Generally speaking, you do not need to clean the DNA libraries if there are off-target products at 700 bp or higher. Amplicons that are within the 700 – 1500 bp range inefficiently cluster on the sequencer flow cell (Illumina sequencers). Therefore, additional cleanup steps are not necessary.

However, high-molecular-weight products need to be taken into consideration during library quantification.

Additional information can be found in the Tapestri Single-Cell DNA Sequencing V2 User Guide.

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