When should I pool my technical replicates?

  • Updated

Tubes need to be pooled after breaking the emulsions following Targeted PCR. Follow the protocol to break the emulsions, increasing the aqueous layer with added nuclease free water, and then pool 42 μL from each aqueous phase to two new 1.5 mL DNA low-bind Eppendorf tubes. Pool contents from tubes 1-4 and 5-8.

Share this article:

Was this article helpful?

0 out of 0 found this helpful

Have more questions? Submit a request