Why is 1 sample partitioned into 8 tubes after Barcoding?

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The volumes of both the Barcoding Oil and emulsions that are generated during the Barcoding program exceed the volume that one 0.2 mL PCR tube can hold.

For that reason, the sample is partitioned into eight individual 0.2 mL tubes and re-pooled subsequently.

The channels leading to the 8 barcoding tubes are connected. Occasionally one or two tubes may be completely empty. This does not result in a loss of cells as the emulsions are distributed to the remaining tubes. 

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