Skip to main content
Mission Bio Support Center Help Center home page Support Center
  • Submit a request
  1. Mission Bio Support Center
  2. Experimental Workflow
  3. FAQs

Why is 1 sample partitioned into 8 tubes after Barcoding?

  • Updated April 08, 2020 13:50

The volumes of both the Barcoding Oil and emulsions that are generated during the Barcoding program exceed the volume that one 0.2 mL PCR tube can hold.

For that reason, the sample is partitioned into eight individual 0.2 mL tubes and re-pooled subsequently.

Share this article:

Was this article helpful?

0 out of 0 found this helpful

Have more questions? Submit a request

Related articles

  • Can I proceed if the volume of the 8 tubes are uneven?
  • What if I load > 4,000 cells per μL onto the cartridge?
  • Can multiple samples be processed simultaneously?
  • What are the DNA concentrations for Tapestri libraries?
  • Why do I have to remove oil from my emulsion tubes?

Articles in this section

  • How do I remove high molecular weight products in my library?
  • What is the recommended storage temperature for primer pools?
  • Why do I have to remove oil from my emulsion tubes?
  • How do I remove excess primer dimers?
  • Can Tapestri Platform detect mitochondrial DNA?
  • Which cell-counting method should I use?
  • How long can I store my emulsions before proceeding?
  • How long can I store Tapestri libraries?
  • What if I load > 4,000 cells per μL onto the cartridge?
  • Why is 1 sample partitioned into 8 tubes after Barcoding?

See more

Privacy Policy Terms of Use Copyright © Mission Bio. All rights reserved.