Emulsions after Encapsulation
Generally, we do not recommend storing the emulsions after Encapsulation for more than a few minutes at 4 °C before proceeding to the Lysis/Digest thermal cycling program.
After the Lysis/Digest thermal cycling program is complete, emulsions may be stored at 4 °C but must be processed within 24 hours (Barcoding).
Emulsions after Barcoding
The eight-tube barcoding emulsions need to be processed immediately, or stored at 4 °C short-term, after the Barcoding program/UV treatment is completed to ensure efficient PCR amplification.
After Targeted PCR (1st PCR), emulsions may be stored at 4 °C for up to 24 hours before breaking them.
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