Mission Bio does not recommend loading fewer than 100,000 cells in 35 μL onto the cartridge (~3,000 cells/μL).
Loading fewer cells than 3,000 cells/μL will result in reduced cell number output that is available for data analysis.
With a minimum of 100,000 cells, the expected cell output is 5 – 10 % (5,000 – 10,000). When loading a lower number of cells, this capture rate may not be achieved.