After the Encapsulation program and Barcoding program complete, the total volume(s) in each collection tube(s) is typically more than 100 μL. In order to thermal-control the emulsion layer (white layer) on the thermal cycler, the emulsion layer must not exceed the 100 μL level of the collection tube, e.g., thermal cyclers typically handle reagent volumes of up to 100 μL.
As a result, we require you to remove oil volume from the bottom of the tube(s). Use only gel loading tips and pipette slowly when removing oil volume from the bottom of the collection tube(s).
After Encapsulation
After Barcoding (1 of 8 tubes shown)