Several reasons exist for invalid targets:
Invalid ID: The format of the ID is invalid. Change the ID to match the required format.
Duplicate Target: Duplicate targets are not allowed. Delete the target.
Invalid Region Target: Region targets are validated to check the chromosome length constraint. Regions with either start or stop values greater than the chromosome length are marked invalid.
Incorrect Target ID Format: If the target ID contains an additional space, it is marked invalid. For example, Chr13: 28674559-28674790 is invalid.
Incorrect Target ID or Name: Target IDs and names do not support commas. If added, they are removed from the targets before creating the panel.
Masked Regions: If the target has many masked regions (Ns in reference fasta for the target), the panel will fail. Avoid adding region targets with these.
Wrong Coordinates: All coordinates must be entered for one of these genome assemblies in one-based format for the hg (GRCh37) or mm10 (GRCm38) genomes.