- Prior to Tapestri loading, resuspend cells in DPBS.
- Prepare the modified Lysis mix:
| Reagent | Volume per reaction (uL) |
|---|---|
| Lysis Buffer | 59 |
| DTT (0.1 M) | 3 |
| Proteinase K (19 mg/mL) | 3 |
| Rev primer pool | 5.1 |
| Total Volume | 70.1 |
- Run encapsulation using standard v3 protocol.
- Run a modified Lysis program on the thermocycler:
| Temperature | Time |
|---|---|
| 50 °C | 6 hours |
| 80 °C | 10 minutes |
| 4 °C | Hold |