Tapestri Designer currently allows usage of predesigned amplicons to create a panel.
For each amplicon, following information should be present:
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Amplicon ID: This is the system generated ID. It is seen in the output files available in the results zip file.
- Chromosome Number: The chromosome number of the amplicon.
- Amplicon Start: The start coordinate of the amplicon.
- Insert Start: The start coordinate of the insert region.
- Insert End: The end coordinate of the insert.
- Amplicon End: The end coordinate of the amplicon
- Genome: The genome ID that the amplicons belong to.
- Forward Primer: The forward primer sequence of the amplicon.
- Reverse Primer: The reverse primer sequence of the amplicon.
Examples:
MYE_v2_CSF3R_36932055 chr1 36932036 36932056 36932276 36932296 hg19 ACAGTCGTCCTCCTGGCTTG CAGACCTATGTGCTCCAGGG
MYE_v2_CSF3R_36932338,chr1,36932317,36932339,36932566,36932591,hg19,AGGTCTCTGAGCTGTTATGGGA,GGCATGTGTGAGGAACATACTAGTG
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The amplicons can be tab separated or comma separated.
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Duplicate amplicons are not allowed.
- Overlapping amplicons are not allowed.
- Amplicon length should be between 139 and 375.
- Primer length should be between 18 and 35.
- The amplicon coordinates should be one-based, fully closed. Otherwise, you may encounter an error "Primer sequences do not match with reference".
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We recommend using [panel-id]-design-summary.csv or the [panel-id]-designSummary.tab from a previously designed custom panel or catalog panel, to reuse the amplicons from those panels, and to avoid coordinates and formatting issues.
- Download sample file template.