What are the ADO rate, doublet rate, and LOD?

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ADO

The allele dropout (ADO) occurs when a sample is genotyped and one or both alleles are not present. Tapestri’s ADO rate is < 10 %.

Doublet Rate

The doublet rate is the fraction of multiple cells encapsulated within a single oil droplet that can impact data analysis and interpretation if not adequately addressed. Tapestri’s doublet rate is < 8 %.

LOD

The limit of detection (LOD) is the lowest quantity or concentration of a component that can be reliably detected with a given analytical method.

For the DNA + Protein assay, the LOD for SNVs* is a 0.1% subclonal population and the LOD for CNV is a 5% subclonal population

For the Measurable Residual Disease (MRD) assay, the LOD for SNVs is a 0.01% subclonal population, assuming a pre-enrichment process. 

 

*The limit of detection for indels and ITDs depends on the length and nature of the variant.  

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