Tapestri Pipeline v2 User Guide
Tapestri DNA Pipeline User Guide
Table of Contents
- Introduction
- Key features
- Main input/output files
- Input files
- Primary output files
- Step 1: Create Tapestri Pipeline account and launch Tapestri Pipeline
- Create a Tapestri Pipeline account
- Add new users to your Tapestri Pipeline account
- Log into your Mission Bio user account
- Launch Tapestri Pipeline
- Step 2: Upload data
- Mission Bio Service Connector
- Drag and drop
- Cloud Connector (beta)
- Step 3: Define the panel
- Step 4: Create samples
- Step 5: Start Tapestri Pipeline
- Step 6: Review and download data
- Appendix
Mission Bio's Tapestri Pipeline allows customers to process single-cell DNA and DNA + Protein sequencing data generated on the Tapestri Platform.
Tapestri Pipeline: From FASTQ to variant calls
Key features
- Streamlined data analysis from FASTQ files to annotated VCF files
- Targeted and accurate SNVs and indel variant calling
- Create multiple data output files that seamlessly plug into Tapestri Insights, the tapestri R package for genotype-to-phenotype multi-omics analysis, and other third-party software packages
Main input/output files
Input files
- FASTQ files in compressed format (fastq.gz) generated from Illumina MiSeq, HiSeq 2500, HiSeq 4000, or NovaSeq 6000 runs
- Panel .zip file from Tapestri Designer in a compressed format that provides a user-defined set of targeted positions, such as the Acute Myeloid Leukemia (AML) panel
- Custom panels?
Primary output files
- LOOM file – Used in Tapestri Insights
- VCF in compressed format (vcf.gz) – Used with third-party software platforms
- Report file in PDF format – Summarizes sequencing metrics and outputs the total cell number
- Barcode read distribution file – Used in the tapestri R package for SNV, CNV, and protein analysis
For a complete list of output files, jump to this section.
Launching Tapestri Pipeline
To create a Mission Bio account, enter your email address and a password with these requirements on Tapestri Portal.
If you have problems creating an account, see this article.
Tapestri DNA Pipeline is only available to customers. Please contact sales@missionbio.com for information on how to access it.
If you are a customer, launch Tapestri Pipeline from Tapestri Portal.
For more information on Tapestri Pipeline, see the Tapestri DNA Pipeline User Guide.
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