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Tapestri Pipeline v2 User Guide

Tapestri DNA Pipeline User Guide

Table of Contents

  1. Introduction
  2. Key features
  3. Main input/output files
    1. Input files
    2. Primary output files
  4. Step 1: Create Tapestri Pipeline account and launch Tapestri Pipeline
    1. Create a Tapestri Pipeline account
    2. Add new users to your Tapestri Pipeline account
    3. Log into your Mission Bio user account
    4. Launch Tapestri Pipeline
  5. Step 2: Upload data
    1. Mission Bio Service Connector
    2. Drag and drop
    3. Cloud Connector (beta)
  6. Step 3: Define the panel
  7. Step 4: Create samples
  8. Step 5: Start Tapestri Pipeline
  9. Step 6: Review and download data
  10. Appendix

 

Mission Bio's Tapestri Pipeline allows customers to process single-cell DNA and DNA + Protein sequencing data generated on the Tapestri Platform. 

Tapestri Pipeline: From FASTQ to variant calls

Key features

  • Streamlined data analysis from FASTQ files to annotated VCF files
  • Targeted and accurate SNVs and indel variant calling
  • Create multiple data output files that seamlessly plug into Tapestri Insights, the tapestri R package for genotype-to-phenotype multi-omics analysis, and other third-party software packages

Main input/output files

Input files

  • FASTQ files in compressed format (fastq.gz) generated from Illumina MiSeq, HiSeq 2500, HiSeq 4000, or NovaSeq 6000 runs
  • Panel .zip file from Tapestri Designer in a compressed format that provides a user-defined set of targeted positions, such as the Acute Myeloid Leukemia (AML) panel
  • Custom panels?

Primary output files

  • LOOM file – Used in Tapestri Insights
  • VCF in compressed format (vcf.gz) – Used with third-party software platforms
  • Report file in PDF format – Summarizes sequencing metrics and outputs the total cell number
  • Barcode read distribution file – Used in the tapestri R package for SNV, CNV, and protein analysis

For a complete list of output files, jump to this section.

Launching Tapestri Pipeline

To create a Mission Bio account, enter your email address and a password with these requirements on Tapestri Portal.

If you have problems creating an account, see this article.

Tapestri DNA Pipeline is only available to customers. Please contact sales@missionbio.com for information on how to access it.

If you are a customer, launch Tapestri Pipeline from Tapestri Portal.

For more information on Tapestri Pipeline, see the Tapestri DNA Pipeline User Guide.

 

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