Use of the new SPARC algorithm for Phylogeny creation
- What it does: SPARC (Single-cell Phylogeny And Reconstruction of Clones) is a computational method for inferring phylogenetic relationships from single-cell DNA sequencing data. It replaces the COMPASS phylogeny tool which was used in the previous version of the software.
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Key Highlights:
- Better phylogeny trees.
- Decreased cell throughput as the cells with a lot of missing data are now called Ambiguous.
- Due to the updated DNA+Protein merge logic retaining more cells, processing the FASTQ again may result in slightly different variants and phylogeny
Addition of cell clustering to Protein UMAP/Protein expression correlation plots
- Protein data is now clustered using unsupervised clustering methods and all the visualizations now show data based on these clusters in the report.