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Sequencing

Explore sequencing of Tapestri libraries, such as supported sequencers and recommendations for sequencing runs.

    1. Mission Bio Support Center
    2. Sequencing
    • User Guides

      • Tapestri Single-cell DNA Sequencing Requirements Guide
      • Tapestri Library Quantification Tool
    • FAQs

      • What sequencing coverage is recommended?
      • What do I do if my sequencing run is overclustered?
      • What do I do if my sequencing run is underclustered?
      • What if my pass-filter read is < 85 % after sequencing?
      • How do I quantify my Tapestri library for sequencing?
      • Can I use NextSeq 500/550 to sequence my libraries?
      • Is the Custom Sequencing Primer required?
      • Can I convert BCL files to FASTQ files?
      • What causes a low % of mapped reads to cells?
      • Can I reduce the DNA library concentration to < 5 nM?
      • How many samples can I pool with the sample indices?
      • Are undetermined FASTQ files required for Pipeline?
      • Which sequencing chemistry do you recommend?
      • What loading concentration should I use?
      • Which sequencer should I use?
      • What concentration of PhiX should I use?
      • Can I pool Tapestri and other libraries on my sequencer?
      • Can I use PE 250 bp sequencing chemistry to improve FLT3-ITD detection?
    • User Guides

      • Tapestri Single-cell DNA Sequencing Requirements Guide
      • Tapestri Library Quantification Tool
    • FAQs

      • What sequencing coverage is recommended?
      • What do I do if my sequencing run is overclustered?
      • What do I do if my sequencing run is underclustered?
      • What if my pass-filter read is < 85 % after sequencing?
      • How do I quantify my Tapestri library for sequencing?
      • Can I use NextSeq 500/550 to sequence my libraries?
      • Is the Custom Sequencing Primer required?
      • Can I convert BCL files to FASTQ files?
      • What causes a low % of mapped reads to cells?
      • Can I reduce the DNA library concentration to < 5 nM?
      • How many samples can I pool with the sample indices?
      • Are undetermined FASTQ files required for Pipeline?
      • Which sequencing chemistry do you recommend?
      • What loading concentration should I use?
      • Which sequencer should I use?
      • What concentration of PhiX should I use?
      • Can I pool Tapestri and other libraries on my sequencer?
      • Can I use PE 250 bp sequencing chemistry to improve FLT3-ITD detection?
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