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  2. Sequencing
  3. FAQs

FAQs

  • What sequencing coverage is recommended?
  • What do I do if my sequencing run is overclustered?
  • What do I do if my sequencing run is underclustered?
  • What if my pass-filter read is < 85 % after sequencing?
  • How do I quantify my Tapestri library for sequencing?
  • Can I use NextSeq 500/550 to sequence my libraries?
  • Is the Custom Sequencing Primer required?
  • Can I convert BCL files to FASTQ files?
  • What causes a low % of mapped reads to cells?
  • Can I reduce the DNA library concentration to < 5 nM?
  • How many samples can I pool with the sample indices?
  • Are undetermined FASTQ files required for Pipeline?
  • Which sequencing chemistry do you recommend?
  • What loading concentration should I use?
  • Which sequencer should I use?
  • What concentration of PhiX should I use?
  • Can I pool Tapestri and other libraries on my sequencer?
  • Can I use PE 250 bp sequencing chemistry to improve FLT3-ITD detection?
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