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  1. Mission Bio Support Center
  2. Sequencing
  3. FAQs

    1. Mission Bio Support Center
    2. Sequencing
    3. FAQs

    FAQs

    • What sequencing coverage is recommended?
    • What do I do if my sequencing run is overclustered?
    • What do I do if my sequencing run is underclustered?
    • What if my pass-filter read is < 85 % after sequencing?
    • How do I quantify my Tapestri library for sequencing?
    • Can I use NextSeq 500/550 to sequence my libraries?
    • Is the Custom Sequencing Primer required?
    • Can I convert BCL files to FASTQ files?
    • What causes a low % of mapped reads to cells?
    • Can I reduce the DNA library concentration to < 5 nM?
    • How many samples can I pool with the sample indices?
    • Are undetermined FASTQ files required for Pipeline?
    • Which sequencing chemistry do you recommend?
    • What loading concentration should I use?
    • Which sequencer should I use?
    • What concentration of PhiX should I use?
    • Can I pool Tapestri and other libraries on my sequencer?
    • Can I use PE 250 bp sequencing chemistry to improve FLT3-ITD detection?
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