FAQs
- What sequencing coverage is recommended?
- What do I do if my sequencing run is overclustered?
- What do I do if my sequencing run is underclustered?
- What if my pass-filter read is < 85 % after sequencing?
- How do I quantify my Tapestri library for sequencing?
- Can I use NextSeq 500/550 to sequence my libraries?
- Is the Custom Sequencing Primer required?
- Can I convert BCL files to FASTQ files?
- What causes a low % of mapped reads to cells?
- Can I reduce the DNA library concentration to < 5 nM?
- How many samples can I pool with the sample indices?
- Are undetermined FASTQ files required for Pipeline?
- Which sequencing chemistry do you recommend?
- What loading concentration should I use?
- Which sequencer should I use?
- What concentration of PhiX should I use?
- Can I pool Tapestri and other libraries on my sequencer?
- Can I use PE 250 bp sequencing chemistry to improve FLT3-ITD detection?