Are Custom Sequencing Primers required?

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V1 chemistry

Yes, the Custom Sequencing Primer is required when sequencing on the following instruments: MiSeq, HiSeq 2500, HiSeq 4000, NextSeq 550, and NovaSeq 6000.
The primer is provided at a stock concentration of 100 µm.
Refer to the appropriate Illumina Custom Primer’s Guide for additional information.

v2 and v3 chemistry

No custom sequencing primers are required. The Tapestri libraries can be sequenced with the standard Illumina primers (Nextera).

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