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Can I run the GE Pipeline with V2 chemistry data?

  • October 02, 2023 05:45

Yes, the GE Pipeline is compatible with V2 and V3 chemistry data.

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  • Does the GE Pipeline go through QC checks initially? Can anything cause the pipeline to fail during the initial stages?
  • Will I see any difference in my result on the web version (GUI) versus the On-premise versions (USB and server)?
  • Can my % edited cells and % edited alleles be directly compared across my samples?
  • What normalization method is used for Protein expression in the “On-target editing status vs. Protein Expression” plot?
  • Why do I see many low-frequency variants in the “On-target variant distribution” plot for my negative control sample?
  • What size distribution of indels does the report show?
  • What are the recommended values/ ranges for the metrics on the Summary tab?
  • What are the definitions of the metrics on the Summary tab?
  • How do I distinguish between variants (e.g., SNVs) present in my cells (prior to editing) and actual edits?
  • Should I analyze a negative control (unedited sample)?

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