Cases of low library PCR yield or excessive primer dimers in the final library (>50%) can sometimes be rescued by recleaning and concentrating the 1st (targeted) PCR product and repeating the library PCR.
This protocol is designed to rescue sporadic low yield and high primer dimer occurrences. If you have repeated low yield or high primer dimer libraries, please reach out to support@missionbio.com for further troubleshooting.