After the Encapsulation program and Barcoding program complete, the total volume(s) in each collection tube(s) is typically more than 100 μL. In order to thermal-control and preserve the emulsion layer (white layer) on the thermal cycler, the total volume of emulsion + oil should be ~100 μL level of the collection tube, and thermal cyclers should be used with 100 μL blocks.
As a result, we require you to remove oil volume from the bottom of the tube(s) such that the volume ~100 uL. Use only gel loading tips and pipette slowly when removing oil volume from the bottom of the collection tube(s). A sample tube with 100 uL of water can be used for reference.
After Barcoding (1 of 8 tubes shown)