How much tissue is needed for nuclei isolation?

Our nuclei protocol typically yields 0.5 x 10⁶ nuclei from frozen sections of brain, breast, colon, lung, and prostate tumors. The protocol requires a minimum tissue volume of ~8 mm³ or tissue weight of ~40 mg to yield at least 500,000 total nuclei. This number of nuclei is larger than needed for one run on the Tapestri Instrument, and the amount of tissue used may be reduced once the user becomes familiar with the protocol and has a good understanding of the nuclei recovery achieved.