What tissues are valid for the Tapestri Platform?

  • Updated

Sample format

  • Cryopreserved samples, such as 10% DMSO + media/FBS
  • Cultured cells
  • Fresh tissue biopsies – Use Nuclei Extraction protocol or publicly available enzymatic dissociation, DNase-free protocols
  • OCT-embedded tissues – Use Nuclei Extraction protocol
  • Lightly fixed cells/nuclei (DNA-only)
    • Alcohol-based fixed samples, such as acetic acid, EtOH, and MeOH (2:1 Alcohol:Acid)
    • Samples fixed with low paraformaldehyde (PFA) <=0.5% w/v 
  • Maximum cell size <30 µm

Tissue types

  • Cell lines, such as K562 and RAJI
  • PBMCs
  • Bone-marrow-derived cells
  • Solid Tumor derived cells
    • Breast, Lung, Colorectal, Prostate, Glioblastoma multiforme, Pancreatic, Metastatic Melanoma
  • Astrocytoma
  • Liver derived nuclei
  • Patient-derived xenograft (PDX) samples, such as mouse
  • Mouse-derived cells
  • Viral-infected cells

Not yet validated

  • Formalin-fixed paraffin-embedded (FFPE) samples
  • Samples fixed in >0.5% Paraformaldehyde (PFA) w/v
  • Frozen cell pellets
  • Circulating tumor cells (CTCs)
  • Sperm cells

Not supported

  • Cell-free DNA (cfDNA)
  • Prokaryotes
  • Whole blood
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