Gene targets only use coding sequence (CDS) regions within that gene. Tapestri Designer resolves the gene to its CDSand then designs amplicons for only those regions. For example, on creating a panel with gene target as NRAS the following regions are used:
|
Chromosome |
Start |
End |
Region |
|
chr1 |
115251155 |
115251275 |
CDS 1 |
|
chr1 |
115252189 |
115252349 |
CDS 2 |
|
chr1 |
115256420 |
115256599 |
CDS 3 |
|
chr1 |
115258670 |
115258781 |
CDS 4 |
The UTRs and introns are not included in the design. To design a panel for the entire gene, add the regions for the UTRs and introns within the gene as region-based targets. For example, to design for the entire NRAS gene provide these targets:
Gene, NRAS, only CDS
Region, chr1:115247084-115250671, 3'UTR 1
Region, chr1:115250671-115250774, Intron 1
Region, chr1:115250774-115250813, 3'UTR 2
Region, chr1:115250813-115251151, Intron 2
Region, chr1:115251275-115252189, Intron 3
Region, chr1:115252349-115256420, Intron 4
Region, chr1:115256599-115258670, Intron 5
Region, chr1:115258798-115259278, Intron 6
Region, chr1:115259278-115259515, 5'UTR