File: barcode.cell.coverage.tsv

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Pipeline V3


In V3 Pipeline, the file has been moved to the Amplicon Performance table on the Advanced tab in the HTML report. The table is sorted by mean reads which shows all low performing amplicons at the top.


Pipeline V2


This file lists each amplicon and the number of mean reads per cell. For each amplicon, it also lists whether the amplicon passed the threshold. The panel performance threshold calculation is the mean of the total mean reads, e.g., the mean of column B * 0.2. If the mean reads for an amplicon is above this number, it passed the threshold and has a value of TRUE and is considered as a good-performing amplicon in cell calling. If it is below this number, the value is FALSE. The percentage of amplicons passing the threshold equals the Panel Uniformity metric.

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