Sample format
- Cryopreserved samples, such as 10% DMSO + media/FBS
- Cultured cells
- Fresh tissue biopsies – Use Nuclei Extraction protocol or publicly available enzymatic dissociation, DNase-free protocols
- OCT-embedded tissues – Use Nuclei Extraction protocol
- Lightly fixed cells/nuclei
- Alcohol-based fixed samples, such as acetic acid, EtOH, and MeOH (2:1 Alcohol:Acid)
- Samples fixed with low paraformaldehyde (PFA) <=0.5% w/v
- Maximum cell size <30 µm
Tissue types
- Cell lines, such as K562 and RAJI
- PBMCs
- Bone-marrow-derived cells
- Solid Tumor derived cells
- Breast, Lung, Colorectal, Prostate, Glioblastoma multiforme, Pancreatic, Metastatic Melanoma
- Astrocytoma
- Liver derived nuclei
- Patient-derived xenograft (PDX) samples, such as mouse
- Mouse-derived cells
- Viral-infected cells
Not yet validated
- Formalin-fixed paraffin-embedded (FFPE) samples
- Samples fixed in >0.5% Paraformaldehyde (PFA) w/v
- Frozen cell pellets
- Circulating tumor cells (CTCs)
- Sperm cells
Not supported
- Cell-free DNA (cfDNA)
- Prokaryotes
- Whole blood