Yes, Tapestri DNA, RNA, and protein libraries may be pooled and sequenced in one sequencing run.
The smaller protein library (~250bp) clusters slightly better than the DNA and RNA libraries (~450bp), and it should be reduced by ~25% volume on patterned flow cells. Also due to the small amplicon size, Protein libraries are not compatible with 2x250 sequencing. RNA and Protein libraires have low R2 diversity, and may need increased Phi-X concentration if sequencing alone.
Please see the Quantification and Pooling tool for additional information.