What concentration of PhiX should I use?

  • Updated

PhiX recommendations are based on Illumina’s recommendations:

MiSeq HiSeq 2500 HiSeq 3000 HiSeq 4000 NextSeq 500/550 Novaseq 6000
5 % 10 % 15 % 15 % 20 %

15 %

Note: Protein libraries have lower diversity than DNA libraries. It is generally recommended to sequence DNA and Protein libraries together to increase diversity. Increased Phi-X is recommended if sequencing Protein libraries alone.   

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