What causes low emulsion quality, how do I identify it?

  • Updated

The two main sources impacting emulsions quality/stability are:

  • Thermal cycling
  • Emulsion handling

Thermal cycling

  • Incorrect PCR tubes used other than the below validated tubes
    • 0.2 mL Axygen MAXYmum Recovery PCR tubes (Axygen, PCR-02-L-C or PCR-02D-L-C)
    • 0.2 mL TempAssure PCR Flex-Free 8-Tube Strips (North America: USA Scient., 1402-4700, color: natural)
    • 0.2 mL 8-Strip Non-Flex PCR Tubes (Europe: Starlab, I1402-3600, color: natural) 
  • Total volume of emulsion + oil greater than or less than ~100 uL
  • PCR tubes are not fully closed
  • No skirt or supporting ‘corner’ PCR tubes used for even heat transfer

 A layer of fluid above the emulsion layer after the targeted PCR (1st PCR) is indicative of a failure mode.


If you notice an aqueous layer on top of the emulsion layers after thermal cycling, contact support@missionbio.com

 Emulsion handling

  • Static electricity on tubes, gloves, or devices
  • Mechanical stress on tubes

Emulsion tubes are very sensitive to uneven heating, physical stress, or harsh handling. Please refer to the User Guides for instructions on the safe-handling of emulsions.

Share this article:

Was this article helpful?

0 out of 0 found this helpful

Have more questions? Submit a request