An increased cell loading concentration, above 4,000 cells/μL for V2 chemistry and 3,000 cells/μL for V3 chemistry, likely leads to a non-poisson loading of cells, which may result in an increased number of drops containing more than one cell (e.g., multiplets).
- The V2 Tapestri Pipeline is optimized to process up to 10,000 barcodes that are associated with cells. An increased number potentially leads to run errors.
- An above-average number of multiplets, more than 8 %, may impact downstream analysis. No trivial approaches exist to assess cell mixing rates.